econofit macro prep deae column  (Bio-Rad)

Journal: STAR Protocols

Article Title: Protocol to detect m 2 2 G modification on single tRNA iso-decoders by immuno-northern blotting

doi: 10.1016/j.xpro.2025.103924

Figure Lengend Snippet: The assembly of the EconoFit DEAE column for tRNA purification (A) Components required for setting up the EconoFit DEAE column. (B) Fully assembled EconoFit DEAE column with all components from (A) properly connected.

Article Snippet: The assembly of the EconoFit DEAE column for tRNA purification (A) Components required for setting up the EconoFit DEAE column. (B) Fully assembled EconoFit DEAE column with all components from (A) properly connected. b. Insert the trimmed 200 μL pipet tip securely into the center of the fingertight fitting. c. Insert a 1 mL syringe into the pipet tip and slowly push down the plunger to remove the storage buffer from the Bio-Rad EconoFit Macro-Prep DEAE 1 mL column ( B).

Techniques: Purification

Journal: STAR Protocols

Article Title: Protocol to detect m 2 2 G modification on single tRNA iso-decoders by immuno-northern blotting

doi: 10.1016/j.xpro.2025.103924

Figure Lengend Snippet: Identification of tRNA-containing elution fractions from the DEAE column A total of 3.4 mg of total RNA was loaded into the column, and 5 μL of each step elution fractions were resolved on the urea-PAGE gel to identify tRNA-containing fractions. Fractions 14–17 were identified as containing tRNA and should be pooled for precipitation. Marker: NEB Low Range ssRNA Ladder + NEB microRNA Marker. FT = flow through, CSB = column stripping buffer.

Article Snippet: The assembly of the EconoFit DEAE column for tRNA purification (A) Components required for setting up the EconoFit DEAE column. (B) Fully assembled EconoFit DEAE column with all components from (A) properly connected. b. Insert the trimmed 200 μL pipet tip securely into the center of the fingertight fitting. c. Insert a 1 mL syringe into the pipet tip and slowly push down the plunger to remove the storage buffer from the Bio-Rad EconoFit Macro-Prep DEAE 1 mL column ( B).

Techniques: Marker, Stripping Membranes

Journal: STAR Protocols

Article Title: Protocol to detect m 2 2 G modification on single tRNA iso-decoders by immuno-northern blotting

doi: 10.1016/j.xpro.2025.103924

Figure Lengend Snippet: The assembly of the EconoFit DEAE column for tRNA purification (A) Components required for setting up the EconoFit DEAE column. (B) Fully assembled EconoFit DEAE column with all components from (A) properly connected.

Article Snippet: EconoFit Macro-Prep DEAE columns , Bio-Rad , 12009274.

Techniques: Purification

Journal: STAR Protocols

Article Title: Protocol to detect m 2 2 G modification on single tRNA iso-decoders by immuno-northern blotting

doi: 10.1016/j.xpro.2025.103924

Figure Lengend Snippet: Identification of tRNA-containing elution fractions from the DEAE column A total of 3.4 mg of total RNA was loaded into the column, and 5 μL of each step elution fractions were resolved on the urea-PAGE gel to identify tRNA-containing fractions. Fractions 14–17 were identified as containing tRNA and should be pooled for precipitation. Marker: NEB Low Range ssRNA Ladder + NEB microRNA Marker. FT = flow through, CSB = column stripping buffer.

Article Snippet: EconoFit Macro-Prep DEAE columns , Bio-Rad , 12009274.

Techniques: Marker, Stripping Membranes

Journal: STAR Protocols

Article Title: Protocol to detect m 2 2 G modification on single tRNA iso-decoders by immuno-northern blotting

doi: 10.1016/j.xpro.2025.103924

Figure Lengend Snippet: The assembly of the EconoFit DEAE column for tRNA purification (A) Components required for setting up the EconoFit DEAE column. (B) Fully assembled EconoFit DEAE column with all components from (A) properly connected.

Article Snippet: The assembly of the EconoFit DEAE column for tRNA purification (A) Components required for setting up the EconoFit DEAE column. (B) Fully assembled EconoFit DEAE column with all components from (A) properly connected. b. Insert the trimmed 200 μL pipet tip securely into the center of the fingertight fitting. c. Insert a 1 mL syringe into the pipet tip and slowly push down the plunger to remove the storage buffer from the Bio-Rad EconoFit Macro-Prep DEAE 1 mL column ( B).

Techniques: Purification

Journal: STAR Protocols

Article Title: Protocol to detect m 2 2 G modification on single tRNA iso-decoders by immuno-northern blotting

doi: 10.1016/j.xpro.2025.103924

Figure Lengend Snippet: Identification of tRNA-containing elution fractions from the DEAE column A total of 3.4 mg of total RNA was loaded into the column, and 5 μL of each step elution fractions were resolved on the urea-PAGE gel to identify tRNA-containing fractions. Fractions 14–17 were identified as containing tRNA and should be pooled for precipitation. Marker: NEB Low Range ssRNA Ladder + NEB microRNA Marker. FT = flow through, CSB = column stripping buffer.

Article Snippet: The assembly of the EconoFit DEAE column for tRNA purification (A) Components required for setting up the EconoFit DEAE column. (B) Fully assembled EconoFit DEAE column with all components from (A) properly connected. b. Insert the trimmed 200 μL pipet tip securely into the center of the fingertight fitting. c. Insert a 1 mL syringe into the pipet tip and slowly push down the plunger to remove the storage buffer from the Bio-Rad EconoFit Macro-Prep DEAE 1 mL column ( B).

Techniques: Marker, Stripping Membranes